Study on islet function of SD rats in second and third trimesters of pregnancy
- VernacularTitle:妊娠中晚期SD大鼠胰岛功能研究
- Author:
huan-zhen, CHEN
;
xiao, WANG
;
feng-ying, LI
;
yun, LIU
;
hong-mei, LONG
;
ling, WU
;
cui-ping, ZHANG
;
guo, LI
;
min, LUO
- Publication Type:Journal Article
- Keywords:
pregnancy;
islet frame;
intraperitoneal glucose tolerance trial;
glucose-stimulated insulin secretion
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the glucose tolerance and ability of insulin secretion in SD rats in second and third trimesters of pregnancy. Methods SD rats with pregnancy of 15 d were selected as experimental group(n=6),and another 6 rats of the same batch without pregnancy were served as controls(n=6).Intraperitoneal glucose tolerance trials(IPGTT) were conducted in these two groups.Rat islets were isolated after in situ collagenase digestion through pancreatic duct perfusion,islet morphology was observed by inverted phase contrast microscope,and insulin secretion was determined by radioimmunoassay. Results It was revealed by IPGTT that the levels of glucose at 30,60,90 and 120 min were significantly lower in experimental group than those in control group(P
