Effects of high concentration of glucose on growth and collagen synthesis of rat skin fibroblast cells
- VernacularTitle:高糖对大鼠皮肤成纤维细胞生长及胶原蛋白合成的影响
- Author:
zai-chao, ZHANG
;
xi-yun, YE
;
xia, LIU
;
yu-jing, WENG
- Publication Type:Journal Article
- Keywords:
skin fibroblast cells;
high glucose;
RT-PCR;
typeⅠ collagen;
type Ⅲ collagen;
matrix metalloproteinase;
tissue inhibitor of metalloproteinase
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different concentrations of glucose on growth and collagen synthesis of rat skin fibroblast cells cultured in vitro. Methods Rat skin fibroblast cells cultured in vitro were treated with glucose of different concentrations (30,35 and 40mmol/L) for 48 h (high glucose group 1,2 and 3). Cell proliferation was detected by MTT method,the hydroxyproline contents in culture media were determined by the commercial kit,and the mRNA expression of typeⅠand Ⅲ procollagen,matrix metalloproteinase-2(MMP-2)and tissue inhibitor of metalloproteinase-2(TIMP-2)were detected by RT-PCR. Cells cultured with 25mmol/L glucose were served as controls. Results With the increase of glucose concentration in culture media,the growth of skin fibroblast cells was significantly inhibited,and the hydroxyproline contents were significantly decreased (P
