Expression of MT-3 mRNA in human esophageal squamous cell carcinoma
- VernacularTitle:人食管鳞状细胞癌细胞株MT-3 mRNA的表达
- Author:
yi, MIAO
;
bao-qing, LI
;
hui-ning, LIU
;
hui, LIU
;
zi-qiang, TIAN
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
cell line;
metallothionein-3;
RT-PCR
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of metallothionein-3 (MT-3)mRNA in human esophageal squamous cell carcinoma. Methods Five cell lines of human esophageal cancer,TE-1,TE-13,TTN,ECA-109 (cell lines of esophageal squamous cell carcinoma) and OE33 (cell lines of esophageal adenocarcinoma),were used in this study. RT-PCR was employed to detect the expression of MT-3 mRNA. Peripheral blood monouclear cells from normal subjects were served as controls. Results Sequencing of RT-PCR product certified the gene of MT-3 mRNA. It was revealed by gel electrophoresis that there was expression of MT-3 mRNA in each cell line. The relative expression of MT-3 mRNA was 0.230?0.023,0.516?0.020,0.140?0.009,0.176?0.015 and 0.085?0.011 in cell lines of TE-1,TE-13,TTN,ECA-109 and OE33,respectively,significantly lower than that in controls (0.762?0.026) (P
