Effects of low molecular heparin on cytokines in invasive pulmonary aspergillosis mice
- VernacularTitle:低分子肝素对侵袭性肺曲霉病小鼠细胞因子的影响
- Author:
ling, RONG
;
xin, ZHOU
;
mu-dan, HE
;
feng, LI
- Publication Type:Journal Article
- Keywords:
invasive pulmonary aspergillosis;
low molecular weight heparin;
cytokine
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of low molecular heparin(LWMH)on cytokines(TNF-?,IL-1? and IL-10)in blood plasma and bronchoalveolar lavage fluid of invasive pulmonary aspergillosis(IPA)mice.Methods The neutropenic IPA mouse models were established by administration of cyclophosphamide for immunologic function inhibition and intranasally challenge with Aspergillus fumigatus conidia(1?106 conidia/mouse).One hundred and twenty mice were randomly divided into 4 groups:normal control,IPA model,normal saline+LWMH and IPA+LWMH group.Normal saline+LWMH group and IPA+LWMH group received LWMH(subcutaneous injection,1 000 IU/kg,qd?2 d).Normal control and IPA model group received normal saline instedad of LWMH.At 4,8,12,24 and 48 h after inoculation,six mice were randomly taken from each group to be sacrificed.ELISA method was used to determine the concentrations of TNF-?,IL-1? and IL-10 in blood plasma and BALF.Results TNF-?,IL-1? and IL-10 in blood plasma and BALF increased significantly several hours after inoculation of conidia in IPA model and IPA+LWMH group.There were significant higher concentrations of TNF-? and IL-1? in blood plasma and BALF in IPA+LWMH group than in IPA model group(P
