Changes of expression of neuronal nitric oxide synthase after sleep deprivation and effects of Xingnaojing on them
- VernacularTitle:睡眠剥夺后神经元型一氧化氮合酶表达的变化及醒脑静对其影响
- Author:
hong-lian, ZHU
;
zhong-xin, ZHAO
- Publication Type:Journal Article
- Keywords:
sleep deprivation;
nitric oxide synthase;
Xingnaojing;
rat
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of expression of neuronal nitric oxide synthase(nNOS) after sleep deprivation(SD) in rats,and to study the effects of Xingnaojing on them. Methods Rapid eye movement(REM) SD models(SD group,n=40) were established by the modified multiple platform methods.The rats in SD group were subjected to drug intervention group(n=20) and non-drug intervention group(n=20).Xingnaojing injection was administered to the drug intervention group.Rats in SD group were deprived of sleep for 1,3,5 and 7 d. The expression of nNOS in brain tissues was detected by immunohistochemistry.The rats without SD were severed as cage control group(n=10) and tank control group(n=10). Results The expression of positive cells of nNOS in brain tissues increased with the time of SD,and Xingnaojing decreased the number of positive cells of nNOS.The area density of positive reaction cells in SD group was significantly higher than those in cage control group and tank control group(P
