Inhibitory effect of lipoic acid on apoptosis of neurons during neuronal aging
- VernacularTitle:硫辛酸对神经细胞老化过程中细胞凋亡的抑制作用
- Author:
yi, ZHANG
;
ling, MIAO
;
li-min, WANG
;
min, SU
;
yan, CAI
- Publication Type:Journal Article
- Keywords:
lipoic acid;
apoptosis;
neuron;
aging
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of lipoic acid on apoptosis of neurons during experimental neuronal aging. Methods The mouse neuroblastoma cells with serum-free culture were served as experimental neuronal aging models.The cells were randomly divided into control group(serum-free culture)and experimental group(100?mol/L lipoic acid in serum-free culture).After cultivating for 5,10 and 15 d,the cell survival rates were detected by MTT method,and the cell apoptosis rates were quantitatively determined using FACS with Annexin V/PI double staining.Results Compared with those in control group,the cell survival rates in experimental group significantly increased[(212.48?12.26)%vs(100.00?14.80)%,(200.55?28.33)%vs(75.45?10.79)%and(96.81?14.50)%vs(28.54?3.14)%,respectively for 5, 10 and 15 d after cultivation](P