Effects of tumor necrosis factor-? on metabolism of skeletal muscle protein in rats with chronic obstructive pulmonary disease and underlying mechanisms
- VernacularTitle:肿瘤坏死因子-?在慢阻肺大鼠骨骼肌蛋白分解代谢中的作用及其机制
- Author:
yue, ZHANG
;
yong, LUO
;
wei-guo, XU
- Publication Type:Journal Article
- Keywords:
chronic obstructive pulmonary disease;
tumor necrosis factor-?;
skeletal muscle protein;
ubiquitin-dependent proteolytic system;
rat
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of tumor necrosis factor-?(TNF-?) on hypermetabolism of skeletal muscle protein in rats with chronic obstructive pulmonary disease(COPD) and explore the mechanisms. Methods Forty-five SD rats were randomly divided into normal control group,COPD group and COPD +TNF-? group(n=15).Rat COPD models were established by passive cigarette smoking in COPD group and COPD +TNF-? group.After dissecting and isolating the extensor digitorium longus(EDL) muscles,the EDL muscles were either cultured with media containing 10 ug/L recombinant rat TNF-? or without TNF-?.The subsequent changes in ubiquitin mRNA and protein levels were determined by real-time quantitative PCR and Western blot,respectively. Results The expressions of ubiquitin mRNA and protein of COPD group and COPD+TNF-? group were higher than those of normal contro1 group(P
