Inhibition effects of antibacterial proteins from Musca domestica larvae on JEC and A_(375) tumour cells
- VernacularTitle:家蝇幼虫抗菌蛋白对JEC和A_(375)肿瘤细胞的抑制作用
- Author:
qi-hui, WAN
;
li-fang, HE
;
hui, LIU
;
xi, ZHANG
;
da-gang, XU
- Publication Type:Journal Article
- Keywords:
Musca domestica larva;
antibacterial protein;
tumor cell;
cell cycle;
cell apoptosis
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibition effects of antibacterial proteins from Musca domestica larvae on JEC and A375 tumour cells. Methods Antibacterial proteins with concentrations of 0.02%,0.1%,0.5%,2.5% and 12.5% were supplied in the culture of JEC and A375 tumour cells in vitro.The cell cycles and apoptosis of JEC and A375 tumour cells were detected by flow cytometry,and the apoptosis index(AI) was measured.The morphology of apoptotic cells was observed with HE stainings and AO staining.The culture without antibacterial proteins was served as control. ResultsThe ratio of apoptosis index/proliferation index(AI/PI) of JEC cells increased with the concentration of antibacterial proteins.The PI and apoptosis rate of 2.5% antibacterial proteins group and 12.5% antibacterial proteins group significantly increased,and G0/G1 significantly decreased.For A375 cells,there were significant differences in G2/M, S,G0/G1,G2/M,AI/PI and PI between 12.5% antibacterial proteins group and control group(P
