Regulatory effects of tuberculin on growth and apoptosis of liver cancer and lung cancer cell lines
- VernacularTitle:结核菌素对肝癌和肺癌肿瘤细胞生长和凋亡的调控作用
- Author:
zi-zhen, ZHANG
;
yan-ying, SHEN
;
xing-zhi, NI
;
xiao-lu, YIN
- Publication Type:Journal Article
- Keywords:
tuberculin;
cancer cell lines;
cell growth;
cell apoptosis
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effects of tuberculin on growth and apoptosis of liver cancer and lung cancer cell lines. Methods HePG2(liver cancer) and A549(lung cancer) cell lines were treated with TB supernatant(TB-SN) with different concentrations. Cell viability was detected by using LIVE/DEAD Viability/Cytotoxicity cell kits including specific fluorescence primer,and cell apoptosis was detected by Vybrant apoptosis assay. Results After treatment with 5% TB-SN for 5 d,cell apoptosis was significantly increased in HePG2 and A549 cell lines.Cell growth of HePG2 and A549 cell lines was inhibited after treatment with TB-SN. Conclusion Tuberculin can induce cell apoptosis and inhibit cell growth of liver cancer and lung cancer cell lines.
