Preliminary phenotype analysis of RIG-Ⅰ knockout mice
- VernacularTitle:RIG-I基因剔除小鼠表型的初步分析
- Author:
yue-ping, SUN
;
li-jun, ZHANG
;
mei, ZHANG
;
yue-e, JIN
;
zi-xing, LIU
;
hong-xin, ZHANG
;
shun-yuan, LU
;
hui, KONG
;
zhu-gang, WANG
- Publication Type:Journal Article
- Keywords:
retinoic acid-inducible gene-Ⅰ;
bacteria infection;
knockout mice
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the biological functions of retinoic acid-inducible gene-I(RIG-I) in vivo through phenotype analysis of RIG-I knockout mice. Methods The gene expression of RIG-Ⅰ in various tissues of mice was examined with Northern blotting and semi-quantitative RT-PCR.The phenotypes observed included body weight measurement,differential count of peripheral blood cells,metabolic parameters measurement and histopathologic examination. ResultsRIG-Ⅰ expressed in various tissues of mice with different levels.No gross developmental abnormalities and expected maturation arrest in granulocytic differentiation were observed in RIG-Ⅰ knockout mice.However,RIG-Ⅰ knockout mice exhibited an unexpected increase in the ratios of neutrophiles to lymphocytes in peripheral blood and increased susceptibility to bacteria infection. Conclusion RIG-Ⅰ may play an important role in immune regulation in mice.
