Effects of active component of Zhimu on APP and BACE1 in HEK293sw cells
- VernacularTitle:知母皂苷元对HEK293sw细胞APP和BACE1的影响
- Author:
rui, ZHANG
;
zi-mei, WANG
;
zong-qin, XIA
;
ya-er, HU
- Publication Type:Journal Article
- Keywords:
active component of Zhimu;
HEK293sw cells;
?-secretase;
amyloid precursor protein
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ZMS,the active component of Zhimu,on amyloid precursor protein(APP) and ?-site APP cleaving enzyme 1(BACE1) in HEK293sw cells. Methods HEK293sw cells were pretreated with different concentrations of ZMS in routine culture medium for 24 h,and then serum-free medium with ZMS was used for further culture for 48 h.Cells were examined for expression of APP and ?-secretase BACE1 with Western blotting and RT-PCR,and were then examined for BACE1 activity with fluorescence method. Results 1 ?mol/L and 10 ?mol/L ZMS significantly decreased the expression of BACE1 mRNA and protein,while had no effect on the expression of APP mRNA and protein.It was indicated by fluorescence analysis that 10 ?mol/L ZMS significantly decreased the ?-secretase activity.Conclusion ZMS significantly decreases the activity and expression of ?-secretase BACE1,while has no effect on the expression of APP in HEK293sw cells.
