Effects of lipopolysaccharide on expression of hypoxia inducible factor-1? and its target gene GLUT-1 in human monocyte lines THP-1 cells
- VernacularTitle:脂多糖对人单核细胞株THP-1细胞HIF-1?及其靶基因GLUT-1表达的影响
- Author:
bei-lei, WANG
;
xiang-rui, WANG
- Publication Type:Journal Article
- Keywords:
lipopolysaccharide;
hypoxia-inducible factor-1?;
glucose transporter-1;
inflammation
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lipopolysaccharide(LPS) on the expression of hypoxia inducible factor-1?(HIF-1?) and its target gene glucose transpoter-1(GLUT-1)in human monocyte lines THP-1. Methods THP-1 cells were stimulated with 1 ?g/mL LPS for 0,2,4,6 or 8 h.The expression of HIF-1? protein of THP-1 cells was detected by Western blotting,and RT-PCR was employed to detect the expression of HIF-1? mRNA and GLUT-1 mRNA.THP-1 cells were exposed to different concentrations of LPS(0,0.01,0.1 and 1 ?g/mL) for 6 h,and the expression of HIF-1? protein of THP-1 cells was detected by Western blotting. Results The expression of HIF-1? protein of THP-1 cells began to increase 2 h after being treated with 1?g/mL LPS,significantly increased after exposure for 4 h(P
