Effects of glutamine on inflammatory cytokine release in murine peritoneal macrophages during sepsis
- VernacularTitle:谷氨酰胺对脓毒症小鼠腹腔巨噬细胞细胞因子分泌的影响
- Author:
yuan, YUAN
;
xue-min, WANG
;
meng-fan, LIANG
;
wei, JIANG
- Publication Type:Journal Article
- Keywords:
glutamine;
macrophage;
cytokine;
heat shock protein
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vivo effects of glutamine(Gln) on inflammatory cytokine release in murine peritoneal macrophages during sepsis. Methods Sixty Kunming mice were randomly divided into sham-operation group(Sham group,n=20),operation control group(Con group,n=20) and Gln-treatment group(Gln group,n=20).Sepsis was induced by cecal ligation and puncture in Gln group and Con group,and Gln(0.75 g/kg) or saline was immediately administered via single tail vein injection.Serum was collected and macrophages were harvested from peritoneal lavage at 6 h in these three groups.Intracellular and serum cytokines of tumor necrosis factor-?(TNF-?),interleukin(IL)-6 and IL-10 were detected by ELISA.The expression of TNF-? mRNA in macrophages was analyzed by RT-PCR,and the expression of heat shock protein(HSP) 72 in macrophages was evaluated by Western blotting. Results Gln group demonstrated significantly lower intracellular TNF-? and IL-6 levels than Con group(P0.05).The serum TNF-? level was significantly lower in Gln group than in Con group(P
