Effects of human retinal pigment epithelium conditioned medium on biological characteristics of human retinal stem cells
- VernacularTitle:人视网膜色素上皮细胞条件培养液对视网膜干细胞生物学特性的影响
- Author:
tao, SUN
;
bi-lian, KE
;
xun, XU
;
qing, GU
- Publication Type:Journal Article
- Keywords:
retina;
stem cell;
cell culture;
differentiation;
conditioned medium
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of human retinal pigment epithelium conditioned medium(HRPE-CM) on the biological characteristics of human retinal stem cells(HRSCs). Methods HRSCs were exposed to HRPE-CM and cultivated in three different cultures,including the control,epidermal growth factor(EGF) + basic fibroblast growth factor(bFGF) and HRPE-CM.Cell counting was performed to explore the effects of different culture media on the proliferation of HRSCs,and their properties as neural stem cells were further identified. Results Compared with control group,HRPE-CM significantly promoted the proliferation of HRSCs(P
