Biochemical characterization and comparison of recombinant RNase HIIa and RNase HIIb from Chlamydia pneumoniae
- VernacularTitle:肺炎衣原体重组的RNase HIIa和RNase HIIb的生化性质鉴定与比较
- Author:
dong-li, PEI
;
jian-hua, LIU
- Publication Type:Journal Article
- Keywords:
C.pneumoniae RNase HIIa;
C.pneumoniae RNase HIIb;
characterization
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone and compare RNase HIIa and RNase HIIb of Chlamydia pneumoniae AR39(CpRNaseHIIa and CpRNaseHIIb). Methods Genes of CpRNase HIIs were amplified with the designed primers.Then,the recombinant plasmids were constructed,and CpRNase HIIs were expressed and purified with pET expression system.The 5′-32P-labeled RNA/DNA substrate and DNA with oligoribonucleotides substrates were prepared to identify characterization of CpRNase HIIs. Results Ribonuclease H activity of both CpRNase HIIa and CpRNase HIIb could cleave oligoribonucleotides strand,generating break with 3′-OH and 5′-phosphate ends.The results showed that there was different biochemical characterization between them. Conclusion CpRNase HIIa and CpRNase HIIb have different functions in C.pneumoniae.
