Expression of P-MLCK in human pulmonary arterial endothelial cell induced by lipopolysaccharide
- VernacularTitle:细菌脂多糖诱导人肺动脉内皮细胞表达P-MLCK
- Author:
min-jia, LIN
;
jian-wen, BAI
;
jin-shi, LI
;
shu-min, XU
- Publication Type:Journal Article
- Keywords:
human pulmonary arterial endothelial cell;
lipopolysaccharide;
phosphorylated myosin light chain kinase
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of phosphorylated myosin light chain kinase(P-MLCK) in human pulmonary arterial endothelial cell(HPAEC) induced by lipopolysaccharide(LPS). Methods HPAECs were cultured in vitro and treated with LPS(2 ?g/mL) and normal saline for 1 h,respectively.Immunofluorescence method and western blotting were used to detect P-MLCK. Results Compared with normal saline group,the number of HPAECs decreased,but the morphology of cells did not change.After treatment of LPS for 30 and 60 min,the expression of P-MLCK in HPAEC increased from 0.41?0.05 to 0.82?0.43 and 1.56?0.07,respectively(P
