Effect of ambroxol chloride on aquaporin-5 expression in lipopolysaccharide-smoking inducible rats
- VernacularTitle:氨溴索对脂多糖-烟雾诱导大鼠水通道蛋白5表达的影响
- Author:
shao-bin, LIU
;
jin-sheng, OU-YANG
;
shao-guang, HUANG
;
huan-ying, WAN
- Publication Type:Journal Article
- Keywords:
aquaporin 5;
tumor necrosis factor;
ambroxol chloride;
chronic bronchitis
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of aquaporin-5(AQP5) in lipo-polysaccharide(LPS)-cigarette smoking inducible SD rats,and the effect of ambroxol chloride(AMB)on its expression. Methods Twenty-one SD rats were randomly divided into three groups: AMB intervention group,model group(LPS-cigarette smoking induction group) and control group.TNF-? was determined from lung homogenate supernatant,bronchial alveolar lavage fluid(BALF) and serum by ELISA.The semi-quantitation of AQP5 transcription and expression were measured by in situ hybridization and immunohistochemistry,respectively. Results TNF-? from lung homogenate supernatant and BALF in model group was more than AMB intervention group and control group(P
