Effects of oxidative stress induced by high glucose in endothelial cells
- VernacularTitle:高浓度葡萄糖在内皮细胞氧化应激中的作用
- Author:
qin-min, GE
;
fan, BIAN
;
qing, SU
- Publication Type:Journal Article
- Keywords:
high glucose;
endothelial cell;
oxidative stress;
reactive oxygen species;
NADPH oxidase
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of oxidative stress in porcine iliac endothelial cells(PIECs) induced by high glucose. Methods After being intervened by high glucose for some time, dihydroethidium (DHE) or dihydrorhodamine 123 (DHR123) was used as a reactive oxygen species(ROS) capture. The mean fluorescent intensity(MFI) of above probes which were the products of intracellular oxidation was detected by fluorescent microscopy and flow cytometry, and the level of ROS was thus measured. With lucigenin as chemiluminescence agent, luminescence changes were detected by chemiluminescence analyzer after addition of NADPH to observe the effect of high glucose on activity of NADPH oxidase(NOX). Results Intracellular MFI was markedly elevated with the concentration of high glucose and time of exposure to high glucose. It was revealed by flow cytometry that the NOX activity was significantly activated compared with normal medium treated PIECs(P
