Immunology research on human fetal liver cells transplantation in treatment of metabolic diseases
- VernacularTitle:人胎肝细胞移植治疗代谢性疾病的免疫学研究
- Author:
jiang-bo, WAN
;
xiao-li, LIU
;
hui, LIANG
- Publication Type:Journal Article
- Keywords:
fetal liver cells;
transplantation;
mucopolysaccharidosis;
immunosuppressive agent
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immunological reaction,serum and liver ?-L-iduronidase(IDUA) activity after human fetal liver cells(FLCs) transplantation therapy on mucopolysaccharidosis(MPS) which attribute to IDUA defect.Methods FLCs were transplanted into IDUA deficiency mice.T cell subsets(CD3,CD4 and CD8) was detected by flow cytometry,while IL-2,TNF-? and IFN-? by ELISA,and serum and liver IDUA activity by fluorospectrophotometer at different stage(before transplantation and 5,10,15 days after transplantation).Results Human FLCs survived in IDUA deficiency mice,bringing elevated serum and liver enzyme activity to receptor.T cell subsets,IL-2,TNF-? and IFN-? was significantly higher 5,10 and 15 days after transplantation than those before transplantation(P
