Mitogen-activated protein kinase pathway in antitumor effect of toremifene
- VernacularTitle:MAPK信号转导通路在托瑞米芬非雌激素抗肿瘤中的作用
- Author:
hong-xia, WANG
;
feng-chun, ZHANG
;
ming-zhu, HUANG
- Publication Type:Journal Article
- Keywords:
toremifene;
breast cancer;
mitogen-activated protein kinase pathway;
estrogen receptor
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the antitumor effect of toremifene on MCF7 cell lines,and investigate the role of mitogen-activated protein kinase pathway. Methods Inhibitory effect of toremifene alone or combined with MEK inhibitor PD98059 on MCF7 cells was measured by SRB test,and that on phosphorylated ERK was detected by Western blotting.Results Toremifene exhibited a concentration-dependent inhibitory effect on the activity of MCF7 cells.Phosphorylated ERK was significantly inhibited by 5,10 and 20 mmol/L toremifene.Combined with PD98059,toremifene had a significantly enhanced cytotoxity effect,which exceeded that of application alone. Conclusion Mitogen-activated protein kinase pathway may play an important role in the antitumor effect of toremifene which is independent of estrogens.Combined with PD98059,the antitumor effect of toremifene can be reinforced,indicating a synergistic effect of these two drugs.
