Effects of preoperatively glutamine administration on rat lung injury induced by intestinal ischemia/reperfusion
- VernacularTitle:谷氨酰胺预处理对大鼠肠缺血再灌注后肺损伤的影响
- Author:
gui-qi, GENG
;
ye-shen, ZHU
;
hong, JIANG
- Publication Type:Journal Article
- Keywords:
glutamine;
heat-shock protein;
lung injury;
ischemia/reperfusion;
rat
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate effects of pretreatment with glutamine on lung injury induced by intestinal ischemia/reperfusion(II-R) in rats. Methods Glutamine or saline were injected through tail vein before the model of intestinal ischemia/reperfusion in rats were established.The gene expression of intercellular adhesion molecule-1(ICAM-1) and heat shock protein-70(HSP-70) were tested with RT-PCR methods.The levels of heat shock protein-70 in the lung were measured with Western Blotting.Myeloperoxidase and malondialdehyde and pathological changes were also measured. Results The gene expression of heat shock protein-70 was enhanced by pretreatment with glutamine,and the level of HSP-70 was parallelly increased.Nevertheless,MPO,MDA and the gene expression of ICAM-1 were inhibited. Conclusion Pretreatment with glutamine can lessen the lung injury induced by intestinal ischemia/reperfusion in rats,the induction of HSP-70 gene may be one of the potential mechanisms.
