Expression of myosin light chain kinase in acute lung injury
- VernacularTitle:肌球蛋白轻链激酶在急性肺损伤模型的表达及意义
- Author:
jin-shi, LI
;
jian-wen, BAI
;
min-jia, LIN
;
dou-xia, ZHANG
- Publication Type:Journal Article
- Keywords:
myosin light chain kinase;
acute lung injury;
inflammation
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inflammation and expression of myosin light chain kinase(MLCK) through establishing acute lung injury animal model of mice induced by lipopolysacchride(LPS), and approach the role of MLCK in the mechanism of acute lung injury.Methods Twenty female BALB/c mice were randomly divided into LPS group(n=10) and control group(n=10).The BALB/c mice of LPS and control groups were induced by 30 ?L 0.9% NaCl via intranasal instillation,while only LPS group was treated with LPS(20 ?g/each mice).The pathology,wet/dry lung weight ratio and the total cell quantitation in bronchoalveolar lavage fluid(BALF)were compared between these two groups.Furthermore,immunohistochemistry assays were used to determine the status of MLCK expression in the lung.And RT-PCR was adopted to determine the status of MLCKmRNA in the lung. Results Compared with the control group,the LPS group showed more serious pulmonary hemorrhage,edema and infiltration of neutrophils, significantly increased water content in the lungs and total cell quantitation in BALF(P
