Inhibition effect of Heme oxygenase-1 activator Copp on irradiation-induced endothelial cell apoptosis
- VernacularTitle:HO-1激动剂Copp对放射所致内皮细胞凋亡的抑制作用
- Author:
jing, YU
;
guo-hua, WU
;
qing, LIN
;
ren-hua, ZHOU
;
dong-qing, LU
;
jia, WANG
;
qian, LIU
;
xiu-yan, FEN
- Publication Type:Journal Article
- Keywords:
radiation therapy;
heme oxygenase-1;
endothelial cells;
apoptosis
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore protective effect of Heme oxygenase-1(HO-1) on irradiation-induced endothelial cell apoptosis.Methods Human endothelial cell line EA.hy926 were administered with or without HO-1 activator Copp and/or HO-1 inhibitor Znpp,respectively.Then,cells were treated with or without 8 Gy radiation.The HO-1 protein expression of cells were assessed with Western blotting and apoptosis of cells treated with irradiation were evaluated with flow cytometry.Moreover,cytochrome C releasing into cytosol were also determined by Western blotting. Results In PBS+R group,HO-1 protein expression of EA.hy926 was low posterior to irradiation.When cells were preconditioned with Copp and/or Znpp,then recieved with 8Gy irradiation,the HO-1 protein expression of EA.hy926 increased significantly in comparision with the PBS+R group(P
