In vitro effect of PTEN gene transfection on growth of human colon cancer cell lines
- VernacularTitle:体外转染PTEN基因对人结肠癌细胞生长的影响
- Author:
bing, WANG
;
rui, TANG
;
wan-tao, CHEN
;
mi-er, JIANG
- Publication Type:Journal Article
- Keywords:
colon neoplasm;
tumor suppressor gene;
PTEN;
gene transfection
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vitro effect of wild-type PTEN gene transfection on the growth of human colon cancer cell lines. Methods With liposome transduction technique,the retrovirus vector pBp-PTEN,which contains wild-type PTEN gene segment and pBabe-puro,was transfected into Lovo,one of the PTEN-deficient human colorectal carcinoma cell lines.After identification by Western blotting,cell growth,cell cycle and apoptosis before and after transfection were studied. Results It was indicated by the cell growth curve that after transfection the curve of the transfection group exhibited a mild tendency with no obvious logarithmic growth phase,and the growth velocity was significantly lower than that of the control group(P
