Isolation and characterization of ?_2m~-/Thy-1~+ bone marrow-derived liver stem cells from cholestatic rats in vitro
- VernacularTitle:大鼠?_2m~-/Thy-1~+骨髓源性肝干细胞的体外分选及鉴定
- Author:
ling, LAN
;
chao, SUN
;
yuan-wen, CHEN
;
ding-guo, LI
- Publication Type:Journal Article
- Keywords:
bone marrow stem cells;
liver stem cell;
magnetic bead;
isolation
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the in vitro isolation of ?2m-/Thy-1+ bone marrow-derived liver stem cells(BDLSCs) which bear double features of stem and liver cells from bone marrow stem cells(BMSCs)as so to provide suitable donor cells for the treatment of liver diseases by cellular transplant. Methods ?2m-/Thy-1+ BDLSCs were isolated by magnetic bead cell sorting(MACS) method from cholestatic rats in vitro,and cell purity was detected using flow cytometry.Liver associated phenotype markers were characterized by RT-PCR and immunofluorescence staining. Results BDLSCs isolated by MACS were purified and viable,and possessed hepatocyte-like features at gene and protein levels. Conclusion ?2m-/Thy-1+ BDLSCs are special subsets of BMSCs which may have promising potentials in the stem cell-based treatment of liver diseases.
