Effects of astragalus polysaccharides on metabolism of collagen in diabetic cardiomyopathy
- VernacularTitle:黄芪多糖对糖尿病心肌胶原代谢的影响
- Author:
wei, CHEN
;
hong-ying, YE
;
mao-hua, YU
- Publication Type:Journal Article
- Keywords:
astragalus polysaccharides;
diabetic cardiomyopathy;
collagen
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of astragalus polysaccharides(APS)on the metabolism of collagen in hamsters with diabetes mellitus(DM) and explore the possible mechanism of APS on diabetic cardiomyopathy. Methods The levels of insulin,C-peptide,myocardial enzymes,glycosylated serum protein(GSP),plasma and myocardial angiotensin(AngⅡ) were tested in APS group(n=15) and DM control group(n=15).Immunohistochemistry was used to measure the expression levels of typeⅠand typeⅢ collagen,activities of matrix metalloproteinases(MMPs) were examined by using zymography,and TNF-? and TGF-? mRNA were detected by RT-PCR. Results Compared with DM control group,levels of GSP,myocardial enzymes and myocardial AngⅡ were much lower in APS group(P0.05).The level of expression of collagen Ⅰ,the ratio of collagen Ⅰ/collagen Ⅲ,and the activity of pro-MMP-2 and MMP-2 in APS group were significantly lower than those in the DM group(P
