Practice of human dermal lymphatic endothelial cell isolation and cryopreserving technique
- VernacularTitle:人真皮淋巴管内皮细胞的分离及冷冻保存
- Author:
zhao-hua, JIANG
;
xue-qing, HU
;
ning-fei, LIU
- Publication Type:Journal Article
- Keywords:
lymphatic endothelial cells;
cell culture;
cell cryopreserving;
cell apoptosis;
flow cytometry
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05). Conclusion Enough high-purified LECs can be isolated by collagenase digestion procedure followed by immunomagnetic beads sorting. Post-thawed endothelial cells are proved to have high vitality and growth potential in vitro without significant morphological changes. Cryopreserved LECs may serve as a cell choice for research of lymphangiogenesis and lymphatic patterning.
