Effects of pioglitazone on angiotensin Ⅱ-induced proliferation and collagen expression of adventitial fibroblasts
- VernacularTitle:吡格列酮调节AngⅡ诱导的血管外膜成纤维细胞增殖及胶原表达
- Author:
jia, ZHANG
;
ping-jin, GAO
;
ning-yuan, FANG
- Publication Type:Journal Article
- Keywords:
adventitial fibroblasts;
AngⅡ;
collagen typeⅠ;
proliferation
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of pioglitazone on angiotensin Ⅱ(AngⅡ) -induced proliferation and collagen typeⅠ expression of adventitial fibroblasts (AF). Methods The AFs were isolated from rat thoracic aorta. MTT colorimetry and flow cytometry were used to study the effects of pioglitazone on proliferation and cell cycles of AF. The expression of collagen typeⅠ regulated by pioglitazone was examined by the method of Western blot. Results Pioglitazone inhibited the proliferation of AF in a dose-dependent manner, and the most marked effect could be observed at the concentration of 10?10-6 mol/L for pioglitazone (P
