Effects of heat stress response on neutrophil apoptosis and respiratory burst function
- VernacularTitle:热应激反应对中性粒细胞凋亡及呼吸爆发功能的影响
- Author:
gang, ZHAO
;
hai, GAO
;
xue-ming, WANG
;
wei, JIANG
- Publication Type:Journal Article
- Keywords:
neutrophil;
apoptosis;
heat stress response;
respiratory burst
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effects of heat stress response on neutrophil apoptosis and respiratory burst function. MethodsNeutrophils from each of 18 healthy volunteers were divided into 3 parts,and one part was served as control group,and the other two parts were induced by heat shock or cadmium chloride for heat stress response and named as heat shock group and cadmium chloride group.The neutrophils were incubated in culture medium.At 0,2,3,4,and 6 h following heat stress induction,the heat shock protein(HSP70) expression and the respiratory burst were detected in the neutrophils respectively by using PCR technique and flow cytometer.Level of apoptosis was observed by immuno-fluorescence and flow cytometer DNA ploid at 24 h after heat stress induction. Results In the heat shock and cadmium chloride groups,HSP70 expression at each time point and cell apoptosis at 24 h were significantly higher than those of control group(P
