Effect of catalpol from Radix Rehmanniae on A?_(25-35)-induced apoptosis of PC12 cells
- VernacularTitle:地黄活性成分梓醇抗A?_(25-35)诱导的PC12细胞凋亡作用
- Author:
jin-hong, WANG
;
rui, ZHANG
;
ya-er, HU
;
zong-qin, XIA
- Publication Type:Journal Article
- Keywords:
active component of Radix Rehmanniae;
catalpol;
PC12 cells;
cell apoptosis;
A?25-35
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of catalpol from Radix Rehmanniae on A?25-35-induced apoptosis of PC12 cells.Methods PC12 cells were routinely cultivated and treated by A?25-35(final concentration,20 ?mol/L) 24 hours after the addition of catalpol or saline.Forty-eight hours later,cells were examined for viability and apoptosis by MTT method and TUNEL method,respectively,while Bax and Bcl-2 mRNA expression were analyzed by semi-quantitive RT-PCR. Results Catalpol could significantly elevate the viability at 1?10-5 mol/L and 1?10-4 mol/L(P
