Graft repair of peripheral nerve defects using small intestinal submucosa with crude Schwann cells
- VernacularTitle:小肠黏膜下层复合雪旺细胞粗制品桥接周围神经缺损
- Author:
xue-tao, XIE
;
chang-qing, ZHANG
;
yan, SU
- Publication Type:Journal Article
- Keywords:
small intestinal submucosa;
Schwann cells;
peripheral nerve defects;
nerve conduit;
rat
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the results of graft repair of peripheral nerve defects using small intestinal submucosa(SIS) with crude Schwann cells. Methods Thirty-six male SD rats were randomly divided into three groups,with 12 in each group.Firstly,12-mm gaps of sciatic nerve were made in rats and then treated respectively with porcine SIS(group A),SIS compounded with crude Schwann cells(group B) and auto-nerve grafting(group C) in the three groups.The grafts were harvested 16 weeks postoperatively to evaluate the results of nerve regeneration through histological examination, triceps surae weight,computerized imaging analysis,Trueblue retrograde tracing and transmission electron microscopy. Results Nerve regeneration was confirmed to have extended through the gaps according to the results of histological examination,Trueblue retrograde tracing and transmission electron microscopy.Furthermore,in the respect of triceps surae weight,quantity of axis cylinder per area and percentage of neural tissue,the results of group B and group C were superior to those of group A with a significantly statistical difference(P0.05). Conclusion SIS compounded crude Schwann cells was able to achieve the outcome of auto-nerve grafting and was a promising replacement graft.
