Effects of high glucose on oxidative stress of human peritoneal mesothelial cells
- VernacularTitle:高糖对人腹膜间皮细胞氧化应激的影响
- Author:
fan, BIAN
;
qin-min, GE
;
ming, LI
;
qing, SU
- Publication Type:Journal Article
- Keywords:
high glucose;
human peritoneal mesothelial cells;
oxidative stress;
reactive oxygen species
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of high glucose on oxidative stress of human peritoneal mesothelial cells(HPMCs).Methods HPMCs were cultured in vitro and identified by immunohistochemistry, and cells of second generation were selected. After HPMCs were treated by glucose with different concentrations for some time, MTT method was employed to detect the cell viability. 2’,7’-dichlorofluorescein diacetate (DCFH-DA) was used as a reactive oxygen species (ROS) capture. The cell viability of HPMCs and ROS level were analysed after being intervened by glucose with different concentrations and for different time. Results Viability of HPMCs was significantly inhibited in a dose-and time-dependent manner by high glucose(P
