Synthesis of glycylproline p-nitroanilide tosylate and its clinical application
- VernacularTitle:甘氨酰脯氨酰对硝基苯胺对甲苯磺酸盐的合成及临床应用
- Author:
li-yun, YAO
;
jian-hua, ZHANG
;
rong, WANG
;
yang, LU
- Publication Type:Journal Article
- Keywords:
glycylproline p-nitroanilide tosylate;
glycylprolyl dipeptidyl-aminopeptidase;
hepatic cancer;
gastric carcinoma
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize glycylproline p-nitroanilide tosylate as a substrate for glycylprolyl dipeptidyl-aminopeptidase(GPDA),and to study its clinical application.Methods The title compound was prepared by DCC-HOBt synthesis.GPDA in human serum was detected by continuous monitoring method using substrate. Results The substrate with purity of 98.5% was obtained.The linearity of the method was up to 358.1 U/L.Intraassay CV and interassay CV of GPDA in diverse serum samples were 3.01% and 5.04%,respectively.It was shown that GPDA level in patients with hepatic cancer was significantly increased,while those in patients with gastric carcinoma and chronic gastritis were significantly decreased compared with that in the control group in clinical detecting(P
