Synergistic effects of 9-cis-retinoic acid and 8-cl-cAMP on apoptosis of lung cancer cells
- VernacularTitle:9-顺式-维甲酸联合8-cl-cAMP对肺癌细胞凋亡的协同作用
- Author:
yi, XIANG
;
di, YAO
;
shao-guang, HUANG
;
huan-ying, WAN
- Publication Type:Journal Article
- Keywords:
lung cancer;
9-cis-retinoic acid;
8-cl-cAMP;
growth inhibition;
apoptosis
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the synergistic effects of 9-cis-retinoic acid(9-cis-RA) and 8-cl-cAMP on growth inhibition and apoptosis induction in H460 and H292 cell lines of non-small-cell lung cancer(NSCLC). Methods Experimental groups included 9-cis-RA groups(1,5,10 and 20 ?mol/L),8-cl-cAMP groups(5,10,20 and 50 ?mol/L),9-cis-RA(5 and 10 ?mol/L) combined with 8-cl-cAMP(10 ?mol/L) groups and blank control group.The cell growth inhibition rates were detected by trypan blue staining,and the apoptosis of H460 and H292 cells were observed by Hoechst33258 fluorescence microscope,DNA gel electrophoresis and flow cytometer(FCM). Results 9-cis-RA inhibited the growth of H460 cells in a time-and dose-dependent manner,and induced the apoptosis of H460 cells(P
