Effects of bFGF on alterations in pathology and immunohistochemistry in rats with diffuse axonal injury
- VernacularTitle:bFGF对弥漫性轴突损伤大鼠病理及免疫组化改变的影响
- Author:
li, WEN
;
jiong, DAI
;
wei-yi, HU
;
shan-quan, LI
- Publication Type:Journal Article
- Keywords:
diffuse axonal injury;
basic fibroblast growth factor;
brain-derived neurotrophic factor;
growth associated protein-43;
glial fibrillary acidic protein;
rat
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the alterations in pathology and immunohistochemistry in rats with diffuse axonal injury(DAI) and the effects from basic fibroblast growth factor(bFGF). Methods A weight-drop device was employed to produce DAI in rats.In the treatment group(n=60),bFGF was injected subdurally and subcortically.Besides,normal control group(n=20) and injury-control group(n=60) were also established.The pathological changes were observed by light microscopy and electromicroscopy,and the expression of brain-derived neurotrophic factor(BDNF),growth associated protein-43(GAP-43) and glial fibrillary acidic protein(GFAP) were also examined by immunohistochemistry. Results Typical pathological changes were observed in the basal portion of pons,corpus callosum and white matter of cerebral hemisphere in the rats with DAI.And an upregulation of GFAP,GAP-43 and BDNF was also found.In the treatment group,better outcomes of pathological changes were observed.bFGF increased the expression of BDNF and GAP-43,while inhibited the immunoreactivity of GFAP. Conclusion Topical application of bFGF can improve brain tissue regeneration and speed function recovery in rats with DAI,though its long-term effect warrants further study.
