?-lactamase production in multidrug-resistant Pseudomonas aeruginosa
- VernacularTitle:多重耐药铜绿假单胞菌产?内酰胺酶研究
- Author:
ying, LIU
;
bei, ZHANG
;
li-song, SHEN
- Publication Type:Journal Article
- Keywords:
multidrug resistant;
Pseudomonas aeruginosa;
?-lactamase
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the production of ?-lactamase in multidrug-resistant Pseudomonas aeruginosa and to guide the proper use of antibiotics in clinical practice. Methods The modified three-dimensional extract test was employed to detect ?-lactamase in 30 multidrug-resistant Pseudomonas aeruginosa strains screened from antimicrobial susceptibility test in our hospital,and isoelectric focusing electrophoresis was performed on the enzyme-producing strains. Results No metalloenzyme was detected in all the 30 strains.Twenty-six strains produced ?-lactamase,among which 25 continuously yielded large amount of AmpC enzyme and the other one both AmpC enzyme and extended-spectrum ?-lactamases(ESBLs).86.7% of multidrug-resistant Pseudomonas aeruginosa produced enzyme. ConclusionThe majority of the multidrug-resistant Pseudomonas aeruginosa in our hospital yielded large amount of AmpC enzyme in a continuous way.The modified three-dimensional extract test can eliminate some interference such as the decrease of outer membrane permeability and overexpression of efflux pump,facilitating the effective and accurate detection of ESBLs in Pseudomonas aeruginosa.
