Relationship Between p53 Gene Mutation and Apoptosis and Cell Ploidy of Ovarian Carcinoma
- VernacularTitle:卵巢癌细胞凋亡和细胞倍性与p53基因突变的关系
- Author:
Yi, SUN
;
Qing, SHI
;
Li, WANG
- Publication Type:Journal Article
- Keywords:
ovarian carcinomaapoptosiscell ploidyp53 gene mutation
- From:Journal of Shanghai Jiaotong University(Medical Science)
2000;20(5):439-441，449
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship among p53 gene mutation, apoptosis and cellploidy as well as to explore the role of mutated p53 gene in tumorigenesis. MethodsA total of 85 speci-mens (20 normal ovarian specimens, 20 ovarian benign tumors, 20 non - metastatic carcinomas and 25metastatic carcinomas) were chosen． p53 gene mutations were detected by PCR- SSCP (single strand con-formation polymorphism). Cell ploidy, apoptosis role of carcinoma cells and cell distribution in each cellstage were detected by flow cytometry. Resultsp53 gene mutation and non- mutation of ovarian carci-noma was 60% and 40% respectively ( P >0.05). The rate of apoptosis of carcinoma cells with mutationof p53 gene (20.25%) was much lower than that without p53 mutation (41.68%, P <0.05). Aneuploidcarcinoma cells had been found in 17/23 carcinoma specimens with p53 gene mutation and 8/22 carcinomaspecimens without p53 mutation ( P < 0.05). Aneuploid carcinoma cells metastasize more easily thandiploid carcinoma cells ( P < 0.05). The rate of apoptosis was not different between aneuploid and diploidpatients. ConclusionMutation of p53 gene decreases the apoptosis rate of ovarian carcinoma and maypromote tumorigenesis. Cell ploidy had a linkage with p53 gene mutation, but not with cell apoptosis.
