Quantitative analysis of cariogenic strains in dental plaque of caries-susceptibile children by real-time PCR
- VernacularTitle:实时PCR技术在龋易感儿童牙菌斑致龋菌定量检测中的应用
- Author:
jing-ping, LIANG
;
yun-tao, JIANG
;
chao-lun, LI
;
wei, JIANG
- Publication Type:Journal Article
- Keywords:
real-time PCR;
dental caries susceptibility;
cariogenic bacteria;
dental plaque
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
8).The amount of the targeted microorganisms(Streptococcus mutans,Streptococcus sobrinus,Actinomyces viscosus and Actinomyces naeslundii) and the total number of bacterial cells were determined by real-time PCR based on SYBR-Green I fluorescence.Results The percentages of the four targeted bacteria in high-caries group were significantly higher than those in caries-free group(P
