Differential gene expression between Streptococcus sobrinus 6715 and its fluoride-resistant strains
- VernacularTitle:远缘链球菌6715耐氟菌株与亲代菌株的基因表达差异
- Author:
wei, JIANG
;
jing-ping, LIANG
;
chao-lun, LI
;
yun-tao, JIANG
;
ming-zhu, ZHANG
- Publication Type:Journal Article
- Keywords:
Streptococcus sobrinus;
fluorideresistant strains;
suppression subtractive hybridization;
gene expression
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the differential gene expression between Streptococcus sobrinus(S.sobrinus) 6715 and its fluoride-resistant strains. Methods The fluoride-resistant strains of S.sobrinus 6715 was induced by increasing the concentration of fluoride step by step.Total RNA of both S.sobrinus 6715 and its fluoride-resistant strains was extracted,mRNA was separated and purificated,and then cDNA was obtained by reversed transcription.Suppression subtractive hybridization(SSH) technology was used to detect the differential gene expression between them.The differential gene expression fragments were cloned and compared with the GenBank by BLAST.Results After comparing with the GenBank by BLAST,it was identified that there were two differential gene expression fragments,fruA and SMU.438c. Conclusion The cDNA subtractive lib of differential gene expression between S.sobrinus 6715 and its fluoride-resistant strains was successfully constructed through SSH,which paves a way for the further study of fluoride-resistant mechanism.
