Construction of linker for activation of T cells gene eukaryotic expression vector in mouse
- VernacularTitle:小鼠T细胞活化衔接因子基因真核表达载体的构建
- Author:
lian-ping, REN
;
xue-jun, GUO
- Publication Type:Journal Article
- Keywords:
linker for activation of T cells;
gene clone;
eukaryotic expression vector;
asthma
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the eukaryotic expression vector of linker for activation of T cells(LAT) gene in mouse. MethodsLAT cDNA of mouse mononuclear cells was amplified with polymerase chain reaction.The fragment was inserted into the eukaryotic expression vector of pCMV-HA plasmid.The recombinant plasmid was verified by DNA sequencing and used to transfect lymphocytes of the asthmatic model. Results The length of amplified fragment was 729 bp.The sequence of LAT gene was confirmed by Genbank.The LAT protein could be detected in the lymphocytes of asthmatic model.Conclusion The recombinant eukaryotic expression vector pCMV-HA-LAT can be successfully constructed.
