Gene expression profiles of peri-implantation endometrium of high responders during controlled ovarian hyperstimulation
- VernacularTitle:卵巢高反应对围着床期子宫内膜基因表达谱的影响
- Author:
Qiu-ju, CHEN
;
Li-nan, CHENG
;
Lu, LI
;
Xiao-hong, GAO
;
Yu, WU
;
Zhao-gui, SUN
;
Jian, WANG
;
Xiao-xi, SUN
- Publication Type:Journal Article
- Keywords:
microarray;
screening;
endometrium;
controlled ovarian hyperstimulation
- From:Journal of Shanghai Jiaotong University(Medical Science)
2009;29(7):833-836,881
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the gene expression profiles of peri-implantation endometrium of high responders during controlled ovarian hyperstimulation. Methods High responders with cancelled embryo-transfer during controlled ovarian hyperstimulation (high responder group, n=4) and healthy fertile volunteers (control group, n=3) were performed endometrial biopsies during peri-implantation. Histologic changes of endometrium were observed by HE staining, genes of differential expression were screened with microarrays Affymetrix U133A 2.0 and identified by Real-time PCR. The biological process analysis was performed by online biological information analysis tool PANTHER. Results The ehdometrium was in mid-secretory phase in control group, while development delay was found in some glandular organs in endometrium of high responder group. Three hundred and sixty-four genes of differential expression were screened, among which 233 were up-regulated genes and 131 were down-regulated genes. OPN, PLA2G2, DPPIV, IGFBP5 and SSAT were identified as endometrial function-related genes, whose Real-time PCR findings were positively correlated to gene signal values detected by microarray(r=0.44, P<0.01). PANTHER analysis indicated that genes of differential expression participated in the biological processes of cytokine signal transduction and immunological regulation. Conclusion Ovarian high response affects the gene expression profiles of peri-implantation endometrium, which may be one of the causes of sub-optimal endometrial receptivity.
