Effects of Celecoxib on Proliferation and Apoptosis of Human Endometrial Adenocarcinoma Cell Line HEC-1B
- VernacularTitle:塞来昔布对人子宫内膜腺癌细胞系HEC-1B增殖和凋亡的影响
- Author:
yi-tao, XIAO
;
lai-min, LUO
;
rui, ZHANG
- Publication Type:Journal Article
- Keywords:
cyclooxygenase-2;
celecoxib;
endometrial adenocarcinoma;
proliferation;
apoptosis
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of cyclooxygenase-2(COX-2) selective inhibitor celecoxib on proliferation and apoptosis of human endometrial adenocarcinoma HEC-1B cell line. Methods Methabenzthiazuron(MTT) assay was used to examine the effects of different concentrations of celecoxib on proliferation of HEC-1B cells.When HEC-1B cells were treated with different concentrations of celecoxib for 24 h,cell cycle and apoptosis were detected by flow cytometry.The expression of COX-2 mRNA was analyzed by RT-PCR. Results MTT results indicated that celecoxib could inhibit HEC-1B cell proliferation in a time-and dose-dependent manner.When HEC-1B cells were treated with different concentrations of celecoxib for 24 h,flow cytometry results showed that the cell percent of(G_(0)/G_(1)) phase increased,S and G_(2)/M phase decreased,and cell apoptosis rate also increased,which was significantly different from that of the control group(P
