Proliferation Effect of Neuropeptide Y-Y1 Receptor Agonist on Cerebrovascular Smooth Muscle Cells
- VernacularTitle:NPY-Y1受体激动剂对SHR脑血管平滑肌细胞的促增殖作用
- Author:
yi, QU
;
feng-tao, WEI
;
ning-yuan, FANG
- Publication Type:Journal Article
- Keywords:
neuropeptide Y-Y1 receptor;
vascular smooth muscle cell;
cell proliferation;
L-type calcium channel antagonist;
signal pathway
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pathway and mechanism of neuropeptide Y(NPY)-Y1 receptor agonist,[Leu~(31),Pro~(34)]-NPY,on the cultured cerebrovascular smooth muscle cells(SMCs) in the spontaneously hypertensive rats(SHR) in vitro. Methods The SMCs of the arteries from the brain of SHR were cultured in vitro.Cell cycle was detected with flow cytometry,and the concentration of free intracellular calcium was examined with laser scanning confocal microscopy. Results The percentages of S stage cells of 10~(-5) mol/L and 10~(-6) mol/L NPY was higher than those of the control group(P
