Constructing Tissue Engineered Cartilage in Vitro Using a Perfusion-Hydrodynamic Pressure Bioreactor
- VernacularTitle:灌流-液压式反应器体外构建组织工程化软骨
- Author:
xiao-jie, LU
;
guang-dong, ZHOU
;
hong, LI
;
hui, CAO
;
xia, LIU
;
kai, LIU
;
lei, CUI
;
wei, LIU
;
yi-lin, CAO
- Publication Type:Journal Article
- Keywords:
perfusion-hydrodynamic pressure bioreactor;
chondrocyte;
tissue engineering;
in vitro construction
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility and efficacy of constructing tissue engineered cartilage in vitro(using) a perfusion-hydrodynamic pressure bioreactor. Methods Chondrocytes isolated from swine's auricular cartilage were seeded onto polyglycolic acid(PGA) to be cultured in a three dimensional environment for 1 week.Then the chondrocyte-polymer constructs were divided into two groups: the experimental group and control group(8 constructs in each group).The experimental group was put into the perfusion-hydrodynamic pressure bioreactor to be cultured for another 3 weeks.The parameters of bioreactor were set as follows: flow rate of 100 mL/min,clockwise and anticlockwise 30 min respectively,on/off 8 h/16h,hydrodynamic pressure of 100 kpa with 0.5 Hz for 4 h/d.The control group was cultured with the routine method.Specimens were harvested and analyzed by gross observation,histology,typeⅡcollagen immunohistochemistry and biochemistry after 4 weeks. Results After 4 weeks,gross observation showed cartilage-like tissue was formed in both groups,and tissue wet weight of experimental group and control group were(191.03?18.55) mg and(130.78?10.33) mg,respectively(P
