Establishment of Human Colon Cancer Multi-Drug Resistant Cell Line LS174T/5-Fu
- VernacularTitle:人结肠癌LS174T/5-Fu多药耐药细胞系的建立
- Author:
jun, TAO
;
peng, XUE
;
yang, YAO
;
hui, ZHAO
- Publication Type:Journal Article
- Keywords:
human colon cancer;
multi-drug resistant;
5-fluorouracil;
LS174T/5-Fu
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a human colon cancer multi-drug resistant cell line LS174T/5-fluorouracil(5-Fu) and to explore its biological characteristics. Methods A resistant human colon cancer line LS174T/5-Fu was established by stepwise increasing concentrations of fluorouracil selection from the parental cell line LS174T.Drug sensitivity was detected by MTT assay,and the changes of biological characteristics were determined by light microscopy,cell counting,flow cytometry and RT-PCR. Results LS174T/5-Fu cell line was developed after 6 months with stable resistance to 5-Fu and a resistance index of 40.24.The cells exhibited cross-resistance to cisplatin,etoposide,doxifluridine and hydeoxycamptothecin.LS174T/5-Fu cells grew more slowly than LS174T cells,which was longer than LS174T cell line.Cell cycle distribution of LS174T/5-Fu cells was changed compared with parental cells.The percentage of cells in G_(1) and S phase was increased,while in G_(2) phase was decreased.The level of MRP mRNA expression was increased according to the concentration of 5-Fu in resistant cell lines. Conclusion LS174T/5-Fu cells is a reliable multi-drug resistant cell subline of human colon cancer.The successful establishment of this cell subline has laid a solid foundation for further study of the multi-drug resistant mechanism of human colon cancer induced by 5-fluorouracil.
