Effects of Epidermal Growth Factor on Cell Proliferation and Cell Cycle of Cultured Bovine Corneal Endothelial Cells in vitro
- VernacularTitle:人表皮生长因子对牛角膜内皮细胞增殖和细胞周期的影响
- Author:
yu-lan, WANG
;
chong, XU
;
yao-hua, SHENG
- Publication Type:Journal Article
- Keywords:
epidermal growth factor;
corneal endothelial cell;
cell culture
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of epidermal growth factor (EGF) on cell proliferation and cell cycle of cultured bovine corneal endothelial cells. Methods Bovine corneal endothelial cells were cultured with different concentrations of hEGF (1, 10 and 100 ng/mL). MTT test was performed to evaluate the cell proliferation. The bovine corneal endothelial cells were divided into two groups:control group (cultured in DMEM) and EGF-stimulated group (cultured in DMEM with EGF). Flow cytometry was performed to determine the cell cycle phases on the third and seventh day. Results Compared with the control, EGF enhanced the cell proliferation in a dose-related response. 10 ng/mL and 100 ng/mL EGF were much more effective than 1 ng/mL.On the third day, S phase cells accounted for 24.5% and G_2-M phase cells 0.08% in the control group,while 24.6% and 0.06%, respectively in the EGF-stimulated group. However, on the seventh day, those came to 20.8% and 0.41% in the control group, and 18.2% and 1.55% in the EGF-stimulated group,indicating a significant change in the cell cycle (P
