Choroidal Neovascularization Model of Rats Induced by Diode Laser
- VernacularTitle:半导体激光诱导大鼠脉络膜新生血管形成
- Author:
hua-ping, LIAO
;
ling, WANG
;
shi-sheng, ZHANG
- Publication Type:Journal Article
- Keywords:
laser;
diode;
choroidal neovascularization;
animal model
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the feasibility of inducing choroidal neovascularization(CNV) in Brown Norway(BN) rats by diode laser with wavelength of 810 nm. Methods Thirty-five BN rats were divided into(7 groups.)In each groups,one rat served as control,while the other four recieved retinal photocoagulation by diode(laser) in two eyes,at a spot size of 75 ?m with a duration of 100 ms and power of 140 mW.On 1,3,7,14,21,28 and 56 days after photocoagulation,the formation and nature process of CNV were observed by fundus fluorescein(angiography)(FFA),indocyaninegreen angiography(ICGA) and light microscopy(LM). Results FFA,ICGA and LM demonstrated that CNV began to form on the seventh day after photocoagulation,increased on the fourteenth day,reached the peak on the twenty-first day and kept stable until the fifty-sixth day. Conclusion Diode Laser can successfully induced CNV in BN rats,and it is an ideal animal model for further study.
