Characteristic Research on the Affinity of vMIP to Combine the Chemokine Receptors on Cell Membrane
- VernacularTitle:vMIP对细胞膜上趋化因子受体亲和力的结合特性研究
- Author:
qing-ling, YANG
;
yong-xing, DING
- Publication Type:Journal Article
- Keywords:
viral macrophage inflammatory protein;
chemokine receptor;
radioligand receptor binding methods
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the combined ability of viral macrophage inflammatory protein (vMIP) to receptors through comparison research of vMIP binding to chemokine receptors of peripheral blood macrophages(PBMCs). Methods Combined ability of vMIP to receptors was measured with radioligand assay and identified with saturation, kinetic and specificity analyses. Results Kd is 11.1 nmol/L to human receptors of PBMC and 14.3 nmol/L to monkey receptors. The IC50 is 3.4 nmol/L to CCR5 and 4.5 nmol/L to CXCR4. Conclusion vMIP has high affinity with receptors and high specificity to CCR5 and CXCR4.This may be of great significance in the prevention and treatment of inflammation and HIV infection.
