Effect of IFN-? on Phenotypic Transition of Human Tenon's Conjunctival Capsular Fibroblast
- VernacularTitle:干扰素-?对人结膜下Tenon’s囊成纤维细胞表型转化的干预
- Author:
bing, XIE
;
wen, YE
;
yi-sheng, ZHONG
;
xi, SHEN
- Publication Type:Journal Article
- Keywords:
human Tenon's conjunctival capsular fibroblast;
interferon-?;
phenotypic transition;
myofibroblast;
transforming growth factor-?1;
?-smooth muscle actin
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of interferon-?(IFN-?)on phenotypic transition of human Tenon’s conjunctival capsular fibroblast(HTCF). Methods Cultured HTCF derived from 4 operated human cataracts was induced for 48 hours in absence or presence of IFN-? and/or transforming growth factor-?1(TGF-?1). Then immunocytochemistry and Western blot technology were used to detect the ?-smooth muscle actin(?-SMA)expression and identificate the cell phenotype. Results In contrast to normal HTCF, IFN-?(10 ng/mL) inhibited the expression of ?-SMA(P
