Transfection and Expression of eNOS Gene on Canine Endothelial Cells
- VernacularTitle:内皮型一氧化氮合酶基因在犬内皮细胞的转染和表达
- Author:
guan-hua, XUE
;
ji-wei, ZHANG
;
hao, ZHANG
;
bai-gen, ZHANG
- Publication Type:Journal Article
- Keywords:
gene;
gene transfect;
endothelial cell
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study endothelial nitric oxide synthase (eNOS) gene transfecting endothelial cells (ECs). Methods eNOS gene was transfected into the steady ECs system by cationic liposomal transduction and check the transfection effect by RT-PCR and immunohistochemistry assay. To test the concentrations of NOS, nitric oxide (NO) and von willebrand factor (vWF) in culture media by colorimetry and ELISA, respectively,and transfected EC function was observed. Results The effect of transfection was satisfactory with RT-PCR products electrophoresis, sequence and immunohistochemistry. The concentrations of NOS and NO in transfected EC culture media increased with time (P
